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ebfp2 sequence  (Addgene inc)


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    Structured Review

    Addgene inc ebfp2 sequence
    Ebfp2 Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ebfp2+sequence/eBFP2+(eBFP2)_CD+(Plasmid+%2366034)/pm41615803-449-12-15
    Average 93 stars, based on 31 article reviews
    ebfp2 sequence - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: Evidence for improved DNA repair in long-lived bowhead whale.
    Article Snippet: Denis Firsanov, Max Zacher, Xiao Tian, Todd L. Sformo, Yang Zhao, Gregory Tombline, J. Yuyang Lu, Zhizhong Zheng, Luigi Perelli, Enrico Gurreri, Li Zhang, Jing Guo, Anatoly Korotkov, Valentin Volobaev, Seyed Ali Biashad, Zhihui Zhang, Johanna Heid, Alexander Y. Maslov, Shixiang Sun, Zhuoer Wu, Jonathan Gigas, Eric C. Hillpot, John C. Martinez, Minseon Lee, Alyssa Williams, Abbey Gilman, Nicholas Hamilton, Ekaterina Strelkova, Ena Haseljic, Avnee Patel, Maggie E. Straight, Nalani Miller, Julia Ablaeva, Lok Ming Tam, Chloé Couderc, Michael R. Hoopmann, Robert L. Moritz, Shingo Fujii, Amandine Pelletier, Dan J. Hayman, Hongrui Liu, Yuxuan Cai, Anthony K. L. Leung, Zhengdong Zhang, C. Bradley Nelson, Lisa M. Abegglen, Joshua D. Schiffman, Vadim N. Gladyshev, Carlo C. Maley, Mauro Modesti, Giannicola Genovese, Mirre J. P. Simons, Jan Vijg5 ✉, Andrei Seluanov1,23 ✉ & Vera Gorbunova1,23 ✉

    Article Title: Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivo.
    Article Snippet: .. The hSyn-eBFP2 plasmid was constructed by PCR amplification and elongation of the eBFP2 sequence from Addgene plasmid 66034 using the following PCR primers: (capitalized sequences anneal to eBFP2 whereas noncapitalized sequences are elongation sequences): eBFP2 Elongation Forward: gaaggtaccggatccgccacGATGGTGAGCAAGGGCGAG; eBFP2 ElongationReverse: tatcgataagcttgatatcgCAGCGAGTCAGTGAGCGAG. .. After elongation and amplification, the eBFP2 insert was assembled into Addgene plasmid 50465 after EcoRI-HF (NEB:R3101s) and BamHI-HF (NEB:R3136s) restriction digest using a Gibson reaction (NEB: E2621S).

    Article Title: Evidence for improved DNA repair in the long-lived bowhead whale
    Article Snippet: The fusion was purchased from Integrated DNA Technologies. .. EBFP2 sequence was derived from eBFP2-N2 plasmid (Addgene #54595). .. Cloning was done with In-fusion Snap assembly kit (Takara 638947), NEBuilder HiFi DNA Assembly (NEB, E5520) and T4 DNA ligase (NEB, M0202).

    Derivative Assay:

    Article Title: Evidence for improved DNA repair in long-lived bowhead whale.
    Article Snippet: Denis Firsanov, Max Zacher, Xiao Tian, Todd L. Sformo, Yang Zhao, Gregory Tombline, J. Yuyang Lu, Zhizhong Zheng, Luigi Perelli, Enrico Gurreri, Li Zhang, Jing Guo, Anatoly Korotkov, Valentin Volobaev, Seyed Ali Biashad, Zhihui Zhang, Johanna Heid, Alexander Y. Maslov, Shixiang Sun, Zhuoer Wu, Jonathan Gigas, Eric C. Hillpot, John C. Martinez, Minseon Lee, Alyssa Williams, Abbey Gilman, Nicholas Hamilton, Ekaterina Strelkova, Ena Haseljic, Avnee Patel, Maggie E. Straight, Nalani Miller, Julia Ablaeva, Lok Ming Tam, Chloé Couderc, Michael R. Hoopmann, Robert L. Moritz, Shingo Fujii, Amandine Pelletier, Dan J. Hayman, Hongrui Liu, Yuxuan Cai, Anthony K. L. Leung, Zhengdong Zhang, C. Bradley Nelson, Lisa M. Abegglen, Joshua D. Schiffman, Vadim N. Gladyshev, Carlo C. Maley, Mauro Modesti, Giannicola Genovese, Mirre J. P. Simons, Jan Vijg5 ✉, Andrei Seluanov1,23 ✉ & Vera Gorbunova1,23 ✉

    Article Title: Evidence for improved DNA repair in the long-lived bowhead whale
    Article Snippet: The fusion was purchased from Integrated DNA Technologies. .. EBFP2 sequence was derived from eBFP2-N2 plasmid (Addgene #54595). .. Cloning was done with In-fusion Snap assembly kit (Takara 638947), NEBuilder HiFi DNA Assembly (NEB, E5520) and T4 DNA ligase (NEB, M0202).

    Plasmid Preparation:

    Article Title: Evidence for improved DNA repair in long-lived bowhead whale.
    Article Snippet: Denis Firsanov, Max Zacher, Xiao Tian, Todd L. Sformo, Yang Zhao, Gregory Tombline, J. Yuyang Lu, Zhizhong Zheng, Luigi Perelli, Enrico Gurreri, Li Zhang, Jing Guo, Anatoly Korotkov, Valentin Volobaev, Seyed Ali Biashad, Zhihui Zhang, Johanna Heid, Alexander Y. Maslov, Shixiang Sun, Zhuoer Wu, Jonathan Gigas, Eric C. Hillpot, John C. Martinez, Minseon Lee, Alyssa Williams, Abbey Gilman, Nicholas Hamilton, Ekaterina Strelkova, Ena Haseljic, Avnee Patel, Maggie E. Straight, Nalani Miller, Julia Ablaeva, Lok Ming Tam, Chloé Couderc, Michael R. Hoopmann, Robert L. Moritz, Shingo Fujii, Amandine Pelletier, Dan J. Hayman, Hongrui Liu, Yuxuan Cai, Anthony K. L. Leung, Zhengdong Zhang, C. Bradley Nelson, Lisa M. Abegglen, Joshua D. Schiffman, Vadim N. Gladyshev, Carlo C. Maley, Mauro Modesti, Giannicola Genovese, Mirre J. P. Simons, Jan Vijg5 ✉, Andrei Seluanov1,23 ✉ & Vera Gorbunova1,23 ✉

    Article Title: Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivo.
    Article Snippet: .. The hSyn-eBFP2 plasmid was constructed by PCR amplification and elongation of the eBFP2 sequence from Addgene plasmid 66034 using the following PCR primers: (capitalized sequences anneal to eBFP2 whereas noncapitalized sequences are elongation sequences): eBFP2 Elongation Forward: gaaggtaccggatccgccacGATGGTGAGCAAGGGCGAG; eBFP2 ElongationReverse: tatcgataagcttgatatcgCAGCGAGTCAGTGAGCGAG. .. After elongation and amplification, the eBFP2 insert was assembled into Addgene plasmid 50465 after EcoRI-HF (NEB:R3101s) and BamHI-HF (NEB:R3136s) restriction digest using a Gibson reaction (NEB: E2621S).

    Article Title: Evidence for improved DNA repair in the long-lived bowhead whale
    Article Snippet: The fusion was purchased from Integrated DNA Technologies. .. EBFP2 sequence was derived from eBFP2-N2 plasmid (Addgene #54595). .. Cloning was done with In-fusion Snap assembly kit (Takara 638947), NEBuilder HiFi DNA Assembly (NEB, E5520) and T4 DNA ligase (NEB, M0202).

    Construct:

    Article Title: Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivo.
    Article Snippet: .. The hSyn-eBFP2 plasmid was constructed by PCR amplification and elongation of the eBFP2 sequence from Addgene plasmid 66034 using the following PCR primers: (capitalized sequences anneal to eBFP2 whereas noncapitalized sequences are elongation sequences): eBFP2 Elongation Forward: gaaggtaccggatccgccacGATGGTGAGCAAGGGCGAG; eBFP2 ElongationReverse: tatcgataagcttgatatcgCAGCGAGTCAGTGAGCGAG. .. After elongation and amplification, the eBFP2 insert was assembled into Addgene plasmid 50465 after EcoRI-HF (NEB:R3101s) and BamHI-HF (NEB:R3136s) restriction digest using a Gibson reaction (NEB: E2621S).

    Polymerase Chain Reaction:

    Article Title: Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivo.
    Article Snippet: .. The hSyn-eBFP2 plasmid was constructed by PCR amplification and elongation of the eBFP2 sequence from Addgene plasmid 66034 using the following PCR primers: (capitalized sequences anneal to eBFP2 whereas noncapitalized sequences are elongation sequences): eBFP2 Elongation Forward: gaaggtaccggatccgccacGATGGTGAGCAAGGGCGAG; eBFP2 ElongationReverse: tatcgataagcttgatatcgCAGCGAGTCAGTGAGCGAG. .. After elongation and amplification, the eBFP2 insert was assembled into Addgene plasmid 50465 after EcoRI-HF (NEB:R3101s) and BamHI-HF (NEB:R3136s) restriction digest using a Gibson reaction (NEB: E2621S).

    Amplification:

    Article Title: Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivo.
    Article Snippet: .. The hSyn-eBFP2 plasmid was constructed by PCR amplification and elongation of the eBFP2 sequence from Addgene plasmid 66034 using the following PCR primers: (capitalized sequences anneal to eBFP2 whereas noncapitalized sequences are elongation sequences): eBFP2 Elongation Forward: gaaggtaccggatccgccacGATGGTGAGCAAGGGCGAG; eBFP2 ElongationReverse: tatcgataagcttgatatcgCAGCGAGTCAGTGAGCGAG. .. After elongation and amplification, the eBFP2 insert was assembled into Addgene plasmid 50465 after EcoRI-HF (NEB:R3101s) and BamHI-HF (NEB:R3136s) restriction digest using a Gibson reaction (NEB: E2621S).



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